CLSI M38 A2 : 2ED 2008

Abstract
Committee Membership
Foreword
Updated Information in This Edition
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Antifungal Agents
  5.1 Source
  5.2 Weighing Antifungal Powders
  5.3 Preparing Stock Solutions
  5.4 Number of Concentrations Tested
  5.5 Selection of Antifungal Agents for Routine
      Testing and Reporting
6 Test Procedures
  6.1 Broth Medium
  6.2 Preparing Diluted Antifungal Agents
  6.3 Inoculum Preparation
  6.4 Inoculating RPMI-1640 Medium
  6.5 Incubation
  6.6 MIC and MEC Reading Results
  6.7 Interpretation of Results
  6.8 Broth Macrodilution Modifications
  6.9 Other Modifications
7 QC
  7.1 Purpose
  7.2 QC Responsibilities
  7.3 Selecting Reference Strains
  7.4 Storing Reference Strains
  7.5 Routine Use of Reference Strains
  7.6 Batch of Medium and Lot of Plasticware Control
  7.7 QC Frequency
  7.8 Other Control Procedures
  7.9 QC Strains
References
Appendix A. MECs of Caspofungin and Anidulafungin
Appendix B. RPMI-1640 Medium
Appendix C. McFarland 0.5 Barium Sulfate Turbidity Standard
Appendix D. Oatmeal Agar
Summary of Delegate Comments and Subcommittee Responses
The Quality Management System Approach
Related CLSI Reference Materials

Describes the selection of antifungal agents, preparation of antifungal stock solutions and dilutions for testing implementation and interpretation of test procedures, and quality control requirements for susceptibility testing of filamentous fungi (moulds) that cause invasive and cutaneous fungal infections.