EN ISO 11267:2014

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and material
6 Apparatus
7 Procedure
8 Calculation and expression of results
9 Validity of the test
10 Statistical analysis
11 Test report
Annex A (informative) - Techniques for rearing
        and breeding of Collembola
Annex B (informative) - Determination of water-holding
        capacity
Annex C (informative) - Guidance on adjustment of pH
        of artificial soil
Annex D (informative) - Extraction and counting of
        Collembola
Bibliography

ISO 11267:2014 specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Folsomia candida Willem by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites of concern and waste materials.Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the soil to be tested and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) are either an uncontaminated soil comparable to the soil to be tested (reference soil) or a standard soil (e.g. artificial soil).ISO 11267:2014 provides information on how to use this method for testing substances under temperate conditions.The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.