This International Standard specifies laboratory procedures for measuring the mineralization and nitrification of nitrogen by the soil microbiota.For investigations on the evaluation of soil quality or on effects of contamination, a procedure is given to measure the rates and extent of N-mineralization in soil or soils of known or unknown quality.For investigation of the potential toxicity of chemicals to N-mineralization in soils, a simple procedure is given which allows the impact of single chemicals to be assessed and provides a basis for comparison of the toxicities of different chemicals.