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  • EN ISO 16050:2011

    Current The latest, up-to-date edition.

    Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products - High-performance liquid chromatographic method (ISO 16050:2003)

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    Published date:  13-07-2011

    Publisher:  Comite Europeen de Normalisation

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    Table of Contents - (Show below) - (Hide below)

    Foreword
    1 Scope
    2 Normative references
    3 Principle
    4 Reagents
    5 Apparatus
    6 Procedure
    7 Calculation of results
    8 Precision
    9 Test report
    Annex A (informative) - Results of
            interlaboratory test
    Bibliography

    Abstract - (Show below) - (Hide below)

    ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram.The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.

    General Product Information - (Show below) - (Hide below)

    Committee CEN/TC 275
    Development Note Supersedes EN 12955. (07/2011)
    Document Type Standard
    Publisher Comite Europeen de Normalisation
    Status Current
    Supersedes

    Standards Referencing This Book - (Show below) - (Hide below)

    ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
    ISO 31-0:1992 Quantities and units Part 0: General principles
    ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
    ISO 5725:1986 Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
    ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
    EN 14123:2007 Foodstuffs - Determination of aflatoxin B1 and the sum of aflatoxin B1, B2, G1 and G2 in hazelnuts, peanuts, pistachios, figs, and paprika powder - High performance liquid chromatographic method with post-column derivatisation and immunoaffinity column cleanup
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