I.S. EN ISO 15216-1:2017
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO 15216-1:2017)
Hardcopy , PDF
31-05-2021
English
01-01-2017
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National Foreword
European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Equipment and consumables
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Precision
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation of reagents
and buffers
Annex C (informative) - Real-time RT-PCR mastermixes and
cycling parameters
Annex D (informative) - Real-time RT-PCR primers and
hydrolysis probes for the detection of HAV, norovirus
GI and GII and mengo virus (process control)
Annex E (informative) - Growth of mengo virus strain MC[0]
for use as a process control
Annex F (informative) - RNA extraction using the NucliSENS[R]
system
Annex G (informative) - Generation of dsDNA control stocks
Annex H (informative) - Generation of EC RNA stocks
Annex I (informative) - Typical optical plate layout
Annex J (informative) - Method validation studies and
performance characteristics
Bibliography
Describes a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces.
| DevelopmentNote |
Supersedes SR CEN ISO/TS 15216-1. (05/2017)
|
| DocumentType |
Standard
|
| Pages |
66
|
| PublisherName |
National Standards Authority of Ireland
|
| Status |
Superseded
|
| SupersededBy | |
| Supersedes |
| Standards | Relationship |
| EN ISO 15216-1:2017 | Identical |
| ISO 15216-1:2017 | Identical |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 20838:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods |
| ISO 5725-2:1994 | Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method |
| ISO 17468:2016 | Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method |
| ISO 22119:2011 | Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
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