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ISO 11268-2:2012

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by

Soil quality — Effects of pollutants on earthworms — Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei

Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Superseded date

17-01-2023

Superseded by

ISO 11268-2:2023

Language(s)

French, English

Published date

29-10-2012

€60.00
Excluding VAT

ISO 11268-2:2012 specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials.

Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil comparable to the soil sample to be tested (reference soil) or a standard soil (e.g. artificial soil).

Information is provided on how to use this method for testing chemicals under temperate as well as under tropical conditions.

The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.

This method does not take into account the persistence of the substance during the test.

DevelopmentNote
Supersedes ISO/DIS 11268-2. (10/2012)
DocumentType
Standard
Pages
21
PublisherName
International Organization for Standardization
Status
Superseded
SupersededBy
Supersedes

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NF ISO 18512 : 2007 SOIL QUALITY - GUIDANCE ON LONG AND SHORT TERM STORAGE OF SOIL SAMPLES
NF ISO 16387 : 2004 SOIL QUALITY - EFFECTS OF POLLUTANTS ON ENCHYTRAEIDAE (ENCHYTRAEUS SP.) - DETERMINATION OF EFFECTS ON REPRODUCTION AND SURVIVAL
05/30135042 DC : DRAFT JUN 2005 ISO/DIS 17512-1 - SOIL QUALITY - AVOIDANCE TEST FOR TESTING THE QUALITY OF SOILS AND EFFECTS OF CHEMICALS ON BEHAVIOUR - PART 1: TEST WITH EARTHWORMS (EISENIA FETIDA AND EISENIA ANDREI)
15/30290691 DC : 0 BS ISO 19204 - SOIL QUALITY - PROCEDURE FOR SITE-SPECIFIC ECOLOGICAL RISK ASSESSMENT OF SOIL CONTAMINATION (TRIAD APPROACH)
UNI EN ISO 15952 : 2011 SOIL QUALITY - EFFECTS OF POLLUTANTS ON JUVENILE LAND SNAILS (HELICIDAE) - DETERMINATION OF THE EFFECTS ON GROWTH BY SOIL CONTAMINATION
BS EN ISO 17402:2011 Soil quality. Requirements and guidance for the selection and application of methods for the assessment of bioavailability of contaminants in soil and soil materials
BS ISO 22030 : 2005 SOIL QUALITY - BIOLOGICAL METHODS CHRONIC TOXICITY IN HIGHER PLANTS
DIN EN ISO 22030:2011-09 Soil quality - Biological methods - Chronic toxicity in higher plants (ISO 22030:2005)
ISO 19204:2017 Soil quality — Procedure for site-specific ecological risk assessment of soil contamination (soil quality TRIAD approach)
BS ISO 11269-2 : 2012 SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 2: EFFECTS OF CONTAMINATED SOIL ON THE EMERGENCE AND EARLY GROWTH OF HIGHER PLANTS
NF ISO 17512-1 : 2009 SOIL QUALITY - AVOIDANCE TEST FOR DETERMINING THE QUALITY OF SOILS AND EFFECTS OF CHEMICALS ON BEHAVIOUR - PART 1: TEST WITH EARTHWORMS (EISENIA FETIDA AND EISENIA ANDREI)
EN ISO 18187:2018 Soil quality - Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis (ISO 18187:2016)
BS ISO 17616:2008 Soil quality. Guidance on the choice and evaluation of bioassays for ecotoxicological characterization of soils and soil materials
I.S. EN ISO 15952:2011 SOIL QUALITY - EFFECTS OF POLLUTANTS ON JUVENILE LAND SNAILS (HELICIDAE) - DETERMINATION OF THE EFFECTS ON GROWTH BY SOIL CONTAMINATION
NF ISO 17616 : 2008 SOIL QUALITY - GUIDANCE FOR THE CHOICE AND EVALUATION OF BIOASSAYS FOR ECOTOXICOLOGICAL CHARACTERIZATION OF SOILS AND SOIL MATERIAL
DIN EN 14735:2005-11 Characterization of waste - Preparation of waste samples for ecotoxicity tests
I.S. EN 14735:2005 CHARACTERIZATION OF WASTE - PREPARATION OF WASTE SAMPLES FOR ECOTOXICITY TESTS
BS EN ISO 11267:2014 Soil quality. Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants
04/30086451 DC : DRAFT JUN 2004 BS ISO 15952 - SOIL QUALITY - EFFECTS OF POLLUTANTS ON JUVENILE LAND SNAILS (HELICIDAE) - DETERMINATION OF THE EFFECTS ON GROWTH BY SOIL CONTAMINATION
DIN EN ISO 11269-1:2013-03 Soil quality - Determination of the effects of pollutants on soil flora - Part 1: Method for the measurement of inhibition of root growth (ISO 11269-1:2012)
17/30352199 DC : 0 BS ISO 15799 - SOIL QUALITY - GUIDANCE ON THE ECOTOXICOLOGICAL CHARACTERIZATION OF SOILS AND SOIL MATERIALS
02/562595 DC : DRAFT JUN 2002 BS ISO 16387 - SOIL QUALITY - EFFECTS OF POLLUTANTS ON ENCHYTRAEIDAE (ENCHYTRAEUS SP.) - DETERMINATION OF EFFECTS ON REPRODUCTION AND SURVIVAL
NF EN ISO 20963 : 2011 SOIL QUALITY - EFFECTS OF POLLUTANTS ON INSECT LARVAE (OXYTHYREA FUNESTA) - DETERMINATION OF ACUTE TOXICITY
UNI EN ISO 22030 : 2011 SOIL QUALITY - BIOLOGICAL METHODS - CHRONIC TOXICITY IN HIGHER PLANTS
10/30197453 DC : 0 BS ISO 11269-1 - SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 1: METHOD FOR THE MEASUREMENT OF INHIBITION OF ROOT GROWTH
10/30197450 DC : DRAFT JUNE 2010 BS ISO 11269-2 - SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 2: EFFECTS OF CONTAMINATED SOILS ON THE EMERGENCE AND EARLY GROWTH OF HIGHER PLANTS
DIN ISO 15800:2004-07 SOIL QUALITY - CHARACTERIZATION OF SOIL WITH RESPECT TO HUMAN EXPOSURE
DIN EN ISO 11269-2:2013-05 SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 2: EFFECTS OF CONTAMINATED SOIL ON THE EMERGENCE AND EARLY GROWTH OF HIGHER PLANTS (ISO 11269-2:2012)
DIN EN ISO 11267 E : 2014 SOIL QUALITY - INHIBITION OF REPRODUCTION OF COLLEMBOLA (FOLSOMIA CANDIDA) BY SOIL CONTAMINANTS (ISO 11267:2014)
DIN ISO 17616:2009-11 SOIL QUALITY - GUIDANCE ON THE CHOICE AND EVALUATION OF BIOASSAYS FOR ECOTOXICOLOGICAL CHARACTERIZATION OF SOILS AND SOIL MATERIALS
ISO 15176:2019 Guidance on characterization of excavated soil and other materials intended for re-use
I.S. EN ISO 18187:2018 SOIL QUALITY - CONTACT TEST FOR SOLID SAMPLES USING THE DEHYDROGENASE ACTIVITY OF ARTHROBACTER GLOBIFORMIS (ISO 18187:2016)
BS ISO 15176:2002 Soil quality. Characterization of excavated soil and other soil materials intended for re-use
I.S. EN 17033:2018 PLASTICS - BIODEGRADABLE MULCH FILMS FOR USE IN AGRICULTURE AND HORTICULTURE - REQUIREMENTS AND TEST METHODS
I.S. EN ISO 17402:2011 SOIL QUALITY - REQUIREMENTS AND GUIDANCE FOR THE SELECTION AND APPLICATION OF METHODS FOR THE ASSESSMENT OF BIOAVAILABILITY OF CONTAMINANTS IN SOIL AND SOIL MATERIALS
EN ISO 18311:2018 Soil quality - Method for testing effects of soil contaminants on the feeding activity of soil dwelling organisms - Bait-lamina test (ISO 18311:2016)
ISO 11267:2014 Soil quality — Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants
DIN ISO 16387:2004-09 SOIL QUALITY - EFFECTS OF POLLUTANTS ON ENCHYTRAEIDAE (ENCHYTRAEUS SP.) - DETERMINATION OF EFFECTS ON REPRODUCTION AND SURVIVAL
DIN ISO 17512-2:2012-12 SOIL QUALITY - AVOIDANCE TEST FOR DETERMINING THE QUALITY OF SOILS AND EFFECTS OF CHEMICALS ON BEHAVIOUR - PART 2: TEST WITH COLLEMBOLANS (FOLSOMIA CANDIDA) (ISO 17512-2:2011)
BS ISO 16387:2004 Soil quality. Effects of pollutants on Enchytraeidae (Enchytraeus sp.). Determination of effects on reproduction and survival
BS EN ISO 15952:2011 Soil quality. Effects of pollutants on juvenile land snails (Helicidae). Determination of the effects on growth by soil contamination
17/30352190 DC : 0 BS ISO 21285 - SOIL QUALITY - INHIBITION OF REPRODUCTION OF SOIL MITE (HYPOASPIS ACULEIFER) BY SOIL CONTAMINANTS
BS ISO 18187 : 2016 SOIL QUALITY - CONTACT TEST FOR SOLID SAMPLES USING THE DEHYDROGENASE ACTIVITY OF ARTHROBACTER GLOBIFORMIS
UNI EN ISO 11269-2 : 2013 SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 2: EFFECTS OF CONTAMINATED SOIL ON THE EMERGENCE AND EARLY GROWTH OF HIGHER PLANTS
06/30103118 DC : DRAFT MAY 2006 ISO 18512 - SOIL QUALITY - GUIDANCE ON LONG AND SHORT TERM STORAGE OF SOIL SAMPLES
10/30197045 DC : 0 BS ISO 17512-2 - SOIL QUALITY - AVOIDANCE TEST FOR DETERMINING THE QUALITY OF SOILS AND EFFECTS OF CHEMICALS ON BEHAVIOUR - PART 2: TEST WITH COLLEMBOLANS (FOLSOMIA CANDIDA)
S.R. CEN TR 16110:2010 CHARACTERIZATION OF WASTE - GUIDANCE ON THE USE OF ECOTOXICITY TESTS APPLIED TO WASTE
I.S. EN ISO 18311:2018 SOIL QUALITY - METHOD FOR TESTING EFFECTS OF SOIL CONTAMINANTS ON THE FEEDING ACTIVITY OF SOIL DWELLING ORGANISMS - BAIT-LAMINA TEST (ISO 18311:2016)
NF ISO 11269-2 : 2012 SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 2: EFFECTS OF CONTAMINATED SOIL ON THE EMERGENCE AND EARLY GROWTH OF HIGHER PLANTS
ISO 17512-1:2008 Soil quality — Avoidance test for determining the quality of soils and effects of chemicals on behaviour — Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei)
EN ISO 15952:2011 Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination (ISO 15952:2006)
EN 17033:2018 Plastics - Biodegradable mulch films for use in agriculture and horticulture - Requirements and test methods
BS EN ISO 18187:2018 Soil quality. Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis
UNI EN ISO 17402 : 2011 SOIL QUALITY - REQUIREMENTS AND GUIDANCE FOR THE SELECTION AND APPLICATION OF METHODS FOR THE ASSESSMENT OF BIOAVAILABILITY OF CONTAMINANTS IN SOIL AND SOIL MATERIALS
ISO 18311:2016 Soil quality Method for testing effects of soil contaminants on the feeding activity of soil dwelling organisms Bait-lamina test
DIN ISO 15799:2004-05 Soil quality - Guidance on the ecotoxicological characterization of soils and soil materials (ISO 15799:2003)
07/30162079 DC : 0 BS ISO/DIS 17512-1 - SOIL QUALITY - AVOIDANCE TEST FOR TESTING THE QUALITY OF SOILS AND EFFECTS OF CHEMICALS ON BEHAVIOUR - PART 1: TEST WITH EARTHWORMS (EISENIA FETIDA AND EISENIA ANDREI)
ISO 17616:2008 Soil quality Guidance on the choice and evaluation of bioassays for ecotoxicological characterization of soils and soil materials
ISO 15800:2003 Soil quality Characterization of soil with respect to human exposure
BS EN ISO 11269-2:2013 Soil quality. Determination of the effects of pollutants on soil flora Effects of contaminated soil on the emergence and early growth of higher plants
17/30352193 DC : 0 BS ISO 21286 - SOIL QUALITY - IDENTIFICATION OF ECOTOXICOLOGICAL TEST SPECIES BY DNA BARCODING
DIN ISO 17512-1:2010-06 SOIL QUALITY - AVOIDANCE TEST FOR DETERMINING THE QUALITY OF SOILS AND EFFECTS OF CHEMICALS ON BEHAVIOUR - PART 1: TEST WITH EARTHWORMS (EISENIA FETIDA AND EISENIA ANDREI)
DIN ISO 22030:2005-11 SOIL QUALITY - BIOLOGICAL METHODS - CHRONIC TOXICITY IN HIGHER PLANTS
DIN EN ISO 11267:2014-07 Soil quality - Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants (ISO 11267:2014)
BS ISO 18512:2007 Soil quality. Guidance on long and short term storage of soil samples
NF EN ISO 22030 : 2011 SOIL QUALITY - BIOLOGICAL METHODS - CHRONIC TOXICITY IN HIGHER PLANTS
DIN ISO 18512:2009-03 SOIL QUALITY - GUIDANCE ON LONG AND SHORT TERM STORAGE OF SOIL SAMPLES
DIN EN ISO 15952:2011-09 SOIL QUALITY - EFFECTS OF POLLUTANTS ON JUVENILE LAND SNAILS (HELICIDAE) - DETERMINATION OF THE EFFECTS ON GROWTH BY SOIL CONTAMINATION
NF ISO 22030 : 2005 SOIL QUALITY - BIOLOGICAL METHODS - CHRONIC TOXICITY IN HIGHER PLANTS
BIS IS 15109-2 : 2013 SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 2: EFFECTS OF CONTAMINATED SOIL ON THE EMERGENCE AND EARLY GROWTH OF HIGHER PLANTS
ISO 15176:2002 Soil quality Characterization of excavated soil and other soil materials intended for re-use
I.S. EN ISO 22030:2011 SOIL QUALITY - BIOLOGICAL METHODS - CHRONIC TOXICITY IN HIGHER PLANTS
ISO 15799:2003 Soil quality Guidance on the ecotoxicological characterization of soils and soil materials
ISO 17402:2008 Soil quality — Requirements and guidance for the selection and application of methods for the assessment of bioavailability of contaminants in soil and soil materials
ISO 11269-1:2012 Soil quality — Determination of the effects of pollutants on soil flora — Part 1: Method for the measurement of inhibition of root growth
ISO 22030:2005 Soil quality Biological methods Chronic toxicity in higher plants
ISO 11269-2:2012 Soil quality — Determination of the effects of pollutants on soil flora — Part 2: Effects of contaminated soil on the emergence and early growth of higher plants
BS EN 14735:2005 Characterization of waste. Preparation of waste samples for ecotoxicity tests
EN ISO 11269-2:2013 Soil quality - Determination of the effects of pollutants on soil flora - Part 2: Effects of contaminated soil on the emergence and early growth of higher plants (ISO 11269-2:2012)
EN ISO 17402:2011 Soil quality - Requirements and guidance for the selection and application of methods for the assessment of bioavailability of contaminants in soil and soil materials (ISO 17402:2008)
EN ISO 22030:2011 Soil quality - Biological methods - Chronic toxicity in higher plants (ISO 22030:2005)
EN ISO 11269-1:2012 Soil quality - Determination of the effects of pollutants on soil flora - Part 1: Method for the measurement of inhibition of root growth (ISO 11269-1:2012)
EN ISO 11267:2014 Soil quality - Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants (ISO 11267:2014)
BS ISO 15799:2003 Soil quality. Guidance on the ecotoxicological characterization of soils and soil materials
BS EN ISO 11269-1:2012 Soil quality. Determination of the effects of pollutants on soil flora Method for the measurement of inhibition of root growth
BS EN ISO 22030:2011 Soil quality. Biological methods. Chronic toxicity in higher plants
ISO/TS 20281:2006 Water quality — Guidance on statistical interpretation of ecotoxicity data
DIN EN ISO 17402:2011-09 Soil quality - Requirements and guidance for the selection and application of methods for the assessment of bioavailability of contaminants in soil and soil materials (ISO 17402:2008)
BS ISO 18311 : 2016 SOIL QUALITY - METHOD FOR TESTING EFFECTS OF SOIL CONTAMINANTS ON THE FEEDING ACTIVITY OF SOIL DWELLING ORGANISMS - BAIT-LAMINA TEST
I.S. EN ISO 11267:2014 SOIL QUALITY - INHIBITION OF REPRODUCTION OF COLLEMBOLA (FOLSOMIA CANDIDA) BY SOIL CONTAMINANTS (ISO 11267:2014)
BS ISO 11269-1 : 2012 SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 1: METHOD FOR THE MEASUREMENT OF INHIBITION OF ROOT GROWTH
03/116408 DC : DRAFT OCT 2003 BS ISO 22030 - SOIL QUALITY - BIOLOGICAL METHODS - CHRONIC TOXICITY IN HIGHER PLANTS
BS ISO 19204:2017 Soil quality. Procedure for site-specific ecological risk assessment of soil contamination (soil quality TRIAD approach)
UNI EN ISO 11269-1 : 2013 SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 1: METHOD FOR THE MEASUREMENT OF INHIBITION OF ROOT GROWTH
ISO 17512-2:2011 Soil quality — Avoidance test for determining the quality of soils and effects of chemicals on behaviour — Part 2: Test with collembolans (Folsomia candida)
I.S. EN ISO 11269-1:2012 SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 1: METHOD FOR THE MEASUREMENT OF INHIBITION OF ROOT GROWTH (ISO 11269-1:2012)
UNE-ISO 16387:2010 Soil quality -- Effects of pollutants on Enchytraeidae (Enchytraeus sp.) -- Determination of effects on reproduction and survival.
UNI EN ISO 11267 : 2014 SOIL QUALITY - INHIBITION OF REPRODUCTION OF COLLEMBOLA (FOLSOMIA CANDIDA) BY SOIL CONTAMINANTS
UNE-CEN/TR 16110:2011 IN Characterization of waste - Guidance on the use of ecotoxicity tests applied to waste
DIN ISO 19204:2015-09 (Draft) SOIL QUALITY - PROCEDURE FOR SITE-SPECIFIC ECOLOGICAL RISK ASSESSMENT OF SOIL CONTAMINATION (SOIL QUALITY TRIAD APPROACH) (ISO 19204:2017)
I.S. EN ISO 11269-2:2013 SOIL QUALITY - DETERMINATION OF THE EFFECTS OF POLLUTANTS ON SOIL FLORA - PART 2: EFFECTS OF CONTAMINATED SOIL ON THE EMERGENCE AND EARLY GROWTH OF HIGHER PLANTS (ISO 11269-2:2012)
ISO 15952:2006 Soil quality Effects of pollutants on juvenile land snails (Helicidae) Determination of the effects on growth by soil contamination
ISO 18512:2007 Soil quality Guidance on long and short term storage of soil samples
CEN/TR 16110:2010 Characterization of waste - Guidance on the use of ecotoxicity tests applied to waste
UNI EN 14735 : 2005 CHARACTERIZATION OF WASTE - PREPARATION OF WASTE SAMPLES FOR ECOTOXICITY TESTS

ISO 10381-6:2009 Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 17616:2008 Soil quality Guidance on the choice and evaluation of bioassays for ecotoxicological characterization of soils and soil materials
ISO 11277:2009 Soil quality Determination of particle size distribution in mineral soil material Method by sieving and sedimentation
ISO 15799:2003 Soil quality Guidance on the ecotoxicological characterization of soils and soil materials
ISO 10390:2005 Soil quality Determination of pH
ISO 10694:1995 Soil quality — Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 15176:2002 Soil quality Characterization of excavated soil and other soil materials intended for re-use
ISO 11268-1:2012 Soil quality — Effects of pollutants on earthworms — Part 1: Determination of acute toxicity to Eisenia fetida/Eisenia andrei
ISO 11260:1994 Soil quality Determination of effective cation exchange capacity and base saturation level using barium chloride solution

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