ISO 12966-4:2015
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 4: Determination by capillary gas chromatography
Hardcopy , PDF
English, French
01-06-2015
09-03-2026
ISO 12966-4:2015 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.
The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources.
This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966.
ISO 12966-4:2015 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.
| Committee |
ISO/TC 34/SC 11
|
| DevelopmentNote |
Supersedes ISO/DIS 12966-4, ISO 15304 and ISO 5508. (06/2015)
|
| DocumentType |
Standard
|
| Pages |
21
|
| ProductNote |
THIS STANDARD ALSO REFERS-ISO 6353
|
| PublisherName |
International Organization for Standardization
|
| Status |
Superseded
|
| SupersededBy | |
| Supersedes |
| Standards | Relationship |
| NF EN ISO 12966-4 : 2015 | Identical |
| NBN EN ISO 12966-4 : 2015 | Identical |
| NEN EN ISO 12966-4 : 2015 | Identical |
| NS EN ISO 12966-4 : 2015 | Identical |
| I.S. EN ISO 12966-4:2015 | Identical |
| PN EN ISO 12966-4 : 2015 | Identical |
| SS-EN ISO 12966-4:2015 | Identical |
| BS EN ISO 12966-4:2015 | Identical |
| EN ISO 12966-4:2015 | Identical |
| DIN EN ISO 12966-4:2015-11 | Identical |
| UNE-EN ISO 12966-4:2015 | Identical |
| DIN EN ISO 12966-1:2015-10 | ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON MODERN GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS (ISO 12966-1:2014) |
| BS EN ISO 12966-1:2014 | Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters Guidelines on modern gas chromatography of fatty acid methyl esters |
| UNE-EN ISO 12966-1:2015 | Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (ISO 12966-1:2014) |
| NS-EN ISO 3961:2025 | Animal and vegetable fats and oils - Determination of iodine value (ISO 3961:2024) |
| CFR 21(PTS100-169) : 0 | FOOD AND DRUGS - FOOD AND DRUG ADMINISTRATION, CHAPTER 1 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PARTS 100-169 |
| ISO 12966-2:2017 | Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 2: Preparation of methyl esters of fatty acids |
| ISO 12966-3:2016 | Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH) |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| ISO 661:2003 | Animal and vegetable fats and oils — Preparation of test sample |
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