ISO/TS 21569-5:2016
Current
The latest, up-to-date edition.
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence
Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users
English, French
02-11-2016
Important note : Users cannot be edited or removed once added to your Multi user PDF.
ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified (GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 base pairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GM plants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).
The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may be suitable also for analysis of other products such as feedstuffs and seeds. The procedure requires the extraction of an adequate quantity and quality of amplifiable DNA from the test sample.
The DNA sequence amplified by the P-FMV element-specific method can be detected in samples which contain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods.
| DocumentType |
Technical Specification
|
| Pages |
10
|
| PublisherName |
International Organization for Standardization
|
| Status |
Current
|
| Standards | Relationship |
| XP ISO/TS 21569-5 : 2017 XP | Identical |
| PD ISO/TS 21569-5:2016 | Identical |
| ISO/IEC 17025:2005 | General requirements for the competence of testing and calibration laboratories |
| ISO 24276:2006 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions |
| ISO 21571:2005 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction |
| ISO 21569:2005 | Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Qualitative nucleic acid based methods |
| ISO 21570:2005 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Quantitative nucleic acid based methods |
| ISO 16577:2016 | Molecular biomarker analysis — Terms and definitions |
Access your standards online with a subscription
Features
-
Simple online access to standards, technical information and regulations.
-
Critical updates of standards and customisable alerts and notifications.
-
Multi-user online standards collection: secure, flexible and cost effective.