ISO/TS 21569-6:2016
Current
The latest, up-to-date edition.
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences
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English, French
02-11-2016
ISO/TS 21569-6:2016 specifies a procedure for the detection of a DNA sequence of the modified cry1Ab/Ac gene and a procedure for the detection of the DNA transition sequence between the maize ubiquitin promoter (Pubi) and the cry1Ab/Ac gene. The modified cry1Ab/Ac gene and the Pubi-cry construct are frequently found in genetically modified Bt plants. Both detection methods are based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out.
ISO/TS 21569-6:2016 is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.
DocumentType |
Technical Specification
|
Pages |
10
|
PublisherName |
International Organization for Standardization
|
Status |
Current
|
Standards | Relationship |
PD ISO/TS 21569-6:2016 | Identical |
ISO/IEC 17025:2005 | General requirements for the competence of testing and calibration laboratories |
ISO 24276:2006 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions |
ISO 21571:2005 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction |
ISO 21569:2005 | Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Qualitative nucleic acid based methods |
ISO 21570:2005 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Quantitative nucleic acid based methods |
ISO 16577:2016 | Molecular biomarker analysis — Terms and definitions |
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