UNI EN 1276 : 2009

1 Scope
2 Normative references
3 Terms and definitions
4 Requirements
5 Test method
  5.1 Principle
  5.2 Materials and reagents
       5.2.1 Test organisms
       5.2.2 Culture media and reagents
  5.3 Apparatus and glassware
  5.4 Preparation of test organism suspensions and product
       test solutions
       5.4.1 Test organism suspensions (test and validation
       suspension)
       5.4.2 Product test solutions
  5.5 Procedure for assessing the bactericidal activity of
       the product
       5.5.1 General
       5.5.2 Dilution-neutralization method
       5.5.3 Membrane filtration method
  5.6 Experimental data and calculation
       5.6.1 Explanation of terms and abbreviations
       5.6.2 Calculation
  5.7 Verification of methodology
       5.7.1 General
       5.7.2 Control of weighted mean counts
       5.7.3 Basic limits
  5.8 Expression of results and precision
       5.8.1 Reduction
       5.8.2 Control of active and non-active product test
             solution
       5.8.3 Limiting test organism and bactericidal
             concentration
       5.8.4 Precision, replicates
  5.9 Interpretation of results - conclusion
       5.9.1 General
       5.9.2 Bactericidal activity for general purposes
       5.9.3 Bactericidal activity for specific purposes
  5.10 Test report
Annex A (informative) - Referenced strains in national
        collections
Annex B (informative) - Neutralizers and rinsing liquids
Annex C (informative) - Graphical representations of
        dilution neutralization method and membrane
        filtration method
Annex D (informative) - Example of a typical test report
        Test results (bactericidal suspension test)
Annex E (informative) - Precision of the test result
Bibliography

Describes a test method and the minimum requirements for bactericidal activity of chemical disinfectant and antiseptic products that form a homogeneous, physically stable preparation when diluted with hard water or - in the case of ready-to-use products - with water.