Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
6 Study design and sampling
7 Selection of strains
8 Preparation of the inoculum
9 Inoculation of the test units
10 Control tests
11 Storage of the test units
12 Analysis of test units
13 Expression of the results
14 Test report
Annex A (informative) - Characterization of the
impact of inter-batch variability in physicchemical
parameters on bacterial growth
Annex B (informative) - Decisions tree to calculate
the minimum number of test units t prepare
for the challenge-test study
Annex C (informative) - Example of protocols to
prepare inoculum
Annex D (informative) - Example applications of
challenge tests designed to estimate the maximum
growth rate, lag time and growth potential
Annex E (informative) - Interpretation of results on
growth potential
Bibliography