ISO 12966-2:2017
Current
The latest, up-to-date edition.
Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 2: Preparation of methyl esters of fatty acids
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English, French
31-03-2017
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ISO 12966-2:2017 specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely:
a) a "rapid" transmethylation procedure under alkaline conditions;
b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions;
c) a BF3 transmethylation procedure;
d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
| DevelopmentNote |
Supersedes ISO 5509. (02/2011) Supersedes ISO/DIS 12966-2. (04/2017)
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| DocumentType |
Standard
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| Pages |
15
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| PublisherName |
International Organization for Standardization
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| Status |
Current
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| Supersedes |
| Standards | Relationship |
| DIN EN ISO 12966-2:2017-08 | Identical |
| NF EN ISO 12966-2 : 2017 | Identical |
| NEN EN ISO 12966-2 : 2011 | Identical |
| NS EN ISO 12966-2 : 2017 | Identical |
| I.S. EN ISO 12966-2:2017 | Identical |
| PN EN ISO 12966-2 : 2017 | Identical |
| SN EN ISO 12966-2 : 2017 | Identical |
| UNI EN ISO 12966-2 : 2011 | Identical |
| NEN-EN-ISO 12966-2:2017 | Identical |
| SS-EN ISO 12966-2:2017 | Identical |
| UNI EN ISO 12966-2:2017 | Identical |
| UNE-EN ISO 12966-2:2017 | Identical |
| BS EN ISO 12966-2:2017 | Identical |
| EN ISO 12966-2:2017 | Identical |
| DIN EN ISO 12966-2:2011-05 | Identical |
| NBN EN ISO 12966-2 : 2011 | Identical |
| 14/30286016 DC : 0 | BS EN ISO 12966-4 - ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF METHYL ESTERS OF FATTY ACIDS - PART 4: CAPILLARY GAS CHROMATOGRAPHIC METHOD |
| 13/30281563 DC : 0 | BS EN ISO 12966-1 - OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON GAS CHROMATOGRAPHY |
| ISO 8312:2015 | Rubber compounding ingredients Stearic acid Definition and test methods |
| DIN EN ISO 12966-4:2015-11 | ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 4: DETERMINATION BY CAPILLARY GAS CHROMATOGRAPHY (ISO 12966-4:2015) |
| DIN EN ISO 12966-1:2015-10 | ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON MODERN GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS (ISO 12966-1:2014) |
| DIN EN ISO 3657:2013-12 | ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF SAPONIFICATION VALUE (ISO 3657:2013) |
| ISO 3657:2013 | Animal and vegetable fats and oils Determination of saponification value |
| UNI EN ISO 3657 : 2013 | ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF SAPONIFICATION VALUE |
| BS ISO 8312:2015 | Rubber compounding ingredients. Stearic acid. Definition and test methods |
| BS EN ISO 12966-1:2014 | Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters Guidelines on modern gas chromatography of fatty acid methyl esters |
| BS EN ISO 12966-4:2015 | Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters Determination by capillary gas chromatography |
| I.S. EN ISO 12966-4:2015 | ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 4: DETERMINATION BY CAPILLARY GAS CHROMATOGRAPHY (ISO 12966-4:2015) |
| I.S. EN ISO 12966-1:2014 | ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON MODERN GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS (ISO 12966-1:2014) |
| BS EN ISO 3657:2013 | Animal and vegetable fats and oils. Determination of saponification value |
| I.S. EN ISO 3657:2013 | ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF SAPONIFICATION VALUE (ISO 3657:2013) |
| UNE-EN ISO 3657:2013 | Animal and vegetable fats and oils - Determination of saponification value (ISO 3657:2013) |
| EN ISO 3657:2013 | Animal and vegetable fats and oils - Determination of saponification value (ISO 3657:2013) |
| ISO 12966-4:2015 | Animal and vegetable fats and oils Gas chromatography of fatty acid methyl esters Part 4: Determination by capillary gas chromatography |
| UNE-EN ISO 12966-1:2015 | Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (ISO 12966-1:2014) |
| EN ISO 12966-4:2015 | Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4: Determination by capillary gas chromatography (ISO 12966-4:2015) |
| ISO 1042:1998 | Laboratory glassware — One-mark volumetric flasks |
| ISO 835:2007 | Laboratory glassware — Graduated pipettes |
| ISO 4799:1978 | Laboratory glassware — Condensers |
| ISO 8655-2:2002 | Piston-operated volumetric apparatus Part 2: Piston pipettes |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| ISO 661:2003 | Animal and vegetable fats and oils — Preparation of test sample |
| ISO 78-2:1999 | Chemistry Layouts for standards Part 2: Methods of chemical analysis |
| ISO 4800:1998 | Laboratory glassware — Separating funnels and dropping funnels |
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