ISO/TS 13136:2012
Current
The latest, up-to-date edition.
Microbiology of food and animal feed — Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens — Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups
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07-11-2012
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ISO/TR 13136:2012 describes the identification of Shiga toxin-producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae; b) the genes associated with the serogroups O157, O111, O26, O103, and O145.
In any case, when one or both of the stx genes is/are detected, the isolation of the strain is attempted.
The isolation of STEC from samples positive for the presence of the genes specifying the serogroups in the scope of this method can be facilitated by using serogroup-specific enrichment techniques (e.g. immunomagnetic separation, IMS).
The protocol uses real-time PCR as the reference technology for detection of the virulence and serogroup-associated genes.
ISO/TR 13136:2012 is applicable to: 1) products intended for human consumption and the feeding of animals; 2) environmental samples in the area of food production and food handling; 3) environmental samples in the area of primary production.
| DocumentType |
Technical Specification
|
| Pages |
22
|
| PublisherName |
International Organization for Standardization
|
| Status |
Current
|
| Standards | Relationship |
| XP CEN ISO/TS 13136 : 2012 XP | Identical |
| NEN NPR CEN ISO/TS 13136 : 2012 | Identical |
| S.R. CEN ISO/TS 13136:2012 | Identical |
| UNI CEN ISO/TS 13136 : 2013 | Identical |
| IS 16987 : 2018 | Identical |
| PD CEN ISO/TS 13136:2012 | Identical |
| CEN ISO/TS 13136:2012 | Identical |
| DIN CEN ISO/TS 13136;DIN SPEC 10794:2013-04 | Identical |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 16140-2:2016 | Microbiology of the food chain — Method validation — Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method |
| ISO/TS 19036:2006 | Microbiology of food and animal feeding stuffs Guidelines for the estimation of measurement uncertainty for quantitative determinations |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 7550:1985 | Laboratory glassware — Disposable micropipettes |
| ISO 20837:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for sample preparation for qualitative detection |
| ISO 20838:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods |
| ISO 16140:2003 | Microbiology of food and animal feeding stuffs Protocol for the validation of alternative methods |
| ISO 16654:2001 | Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157 |
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