EN ISO 16050:2011
Current
The latest, up-to-date edition.
Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products - High-performance liquid chromatographic method (ISO 16050:2003)
13-07-2011
Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation of results
8 Precision
9 Test report
Annex A (informative) - Results of
interlaboratory test
Bibliography
ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram.The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.
| Committee |
CEN/TC 275
|
| DevelopmentNote |
Supersedes EN 12955. (07/2011)
|
| DocumentType |
Standard
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Current
|
| Supersedes |
| Standards | Relationship |
| ISO 16050:2003 | Identical |
| NF EN ISO 16050 : 2011 | Identical |
| NS EN ISO 16050 : 2011 | Identical |
| DIN EN ISO 16050:2011-09 | Identical |
| NEN EN ISO 16050 : 2011 | Identical |
| NBN EN ISO 16050 : 2011 | Identical |
| PN EN ISO 16050 : 2011 | Identical |
| UNI EN ISO 16050 : 2011 | Identical |
| SS-EN ISO 16050 : 2011 | Identical |
| UNE-EN ISO 16050:2011 | Identical |
| I.S. EN ISO 16050:2011 | Identical |
| BS EN ISO 16050:2011 | Identical |
| SN EN ISO 16050:2011 | Identical |
| ISO 5725-1:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions |
| ISO 31-0:1992 | Quantities and units Part 0: General principles |
| ISO 5725-2:1994 | Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method |
| ISO 5725:1986 | Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN 14123:2007 | Foodstuffs - Determination of aflatoxin B1 and the sum of aflatoxin B1, B2, G1 and G2 in hazelnuts, peanuts, pistachios, figs, and paprika powder - High performance liquid chromatographic method with post-column derivatisation and immunoaffinity column cleanup |
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