• EN ISO 4833-1:2013

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

    Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique (ISO 4833-1:2013)

    Available format(s): 

    Superseded date:  21-01-2022

    Language(s): 

    Published date:  04-09-2013

    Publisher:  Comite Europeen de Normalisation

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    Table of Contents - (Show below) - (Hide below)

    Foreword
    1 Scope
    2 Normative references
    3 Terms and definitions
    4 Principle
    5 Culture media and diluents
    6 Apparatus
    7 Sampling
    8 Preparation of test sample
    9 Procedure
    10 Expression of results
    11 Test report
    Annex A (informative) - Use of the critical difference
            for the interpretation of results
    Bibliography

    Abstract - (Show below) - (Hide below)

    ISO 4833-1:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption and for animal feed; b) environmental samples in the area of food and feed production and handling.ISO 4833-1:2013 is applicable to: 1) products that require a reliable count when a low limit of detection is specified (below 102/g or 102/ml for liquid samples or below 103/g for solid samples); 2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp.The applicability of ISO 4833-1:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively.For some matrices, the method specified in ISO 4833-1:2013 can give different results to those obtained using the method specified in ISO 4833‑2.

    General Product Information - (Show below) - (Hide below)

    Committee CEN/TC 463
    Development Note Supersedes PREN ISO 4833-1. Together with EN ISO 4833-2, it supersedes EN ISO 4833. (09/2013)
    Document Type Standard
    Publisher Comite Europeen de Normalisation
    Status Superseded
    Superseded By
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    DIN EN 16939 E : 2017 ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY
    BS EN 16939:2017 Animal feeding stuffs. Methods of sampling and analysis. Detection of tylosin, spiramycin and virginiamycin. Thin Layer Chromatography and bioautography
    DIN EN 16936:2016-01 (Draft) ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - SCREENING ON THE ANTIBIOTICS TYLOSIN, VIRGINIAMYCIN, SPIRAMYCIN, BACITRACIN-ZINC AND AVOPARCIN AT SUB-ADDITIVE LEVELS IN COMPOUND FEED BY A MICROBIOLOGICAL PLATE TEST
    I.S. EN 16939:2017 ANIMAL FEEDING STUFFS: METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY
    VDI 4066 Blatt 4:2016-04 Hygiene requirements for the production and filling of dairy products without recontamination
    BS EN 16936:2017 Animal feeding stuffs. Methods of sampling and analysis. Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
    DIN EN 16936 E : 2017 ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - SCREENING ON THE ANTIBIOTICS TYLOSIN, VIRGINIAMYCIN, SPIRAMYCIN, BACITRACIN-ZINC AND AVOPARCIN AT SUB-ADDITIVE LEVELS IN COMPOUND FEED BY A MICROBIOLOGICAL PLATE TEST
    DIN EN 16939:2015-12 (Draft) ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY
    UNE-EN 16936:2017 Animal feeding stuffs: Methods of sampling and analysis - Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
    I.S. EN 16936:2017 ANIMAL FEEDING STUFFS: METHODS OF SAMPLING AND ANALYSIS - SCREENING ON THE ANTIBIOTICS TYLOSIN, VIRGINIAMYCIN, SPIRAMYCIN, BACITRACIN-ZINC AND AVOPARCIN AT SUB-ADDITIVE LEVELS IN COMPOUND FEED BY A MICROBIOLOGICAL PLATE TEST
    EN 16936:2017 Animal feeding stuffs: Methods of sampling and analysis - Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
    EN 16939:2017 Animal feeding stuffs: Methods of sampling and analysis - Detection of tylosin, spiramycin and virginiamycin - Thin Layer Chromatography and bioautography
    DIN EN 16939:2017-10 ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY

    Standards Referencing This Book - (Show below) - (Hide below)

    ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
    ISO 835:2007 Laboratory glassware — Graduated pipettes
    ISO/TS 11139:2006 Sterilization of health care products Vocabulary
    ISO 8655-2:2002 Piston-operated volumetric apparatus Part 2: Piston pipettes
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